Determination of Total and Separate Stereoisomers of Diaminopimelic Acid in Five Species and Mixed Rumen Bacteria Using High-Performance Liquid Chromatography
نویسندگان
چکیده
Total (with one peak) and separate three stereoisomers of 2,6-diaminopimelic acid (DAP) in hydrolysed bacterial species and mixed rumen bacteria were determined. The contents of the total DAP of the hydrolysed mixed rumen bacteria collected from sheep and cattle on the average were 30.1 and 12.3 μmol g bacterial DM . The contents of mesoand LLDAP in the hydrolysed mixed rumen bacteria collected from -1 sheep on the average were 26.2 and 3.8 and those collected from cattle were 8.0 and 4.1 μmol g bacterial DM , respectively. DD-DAP was not detected in the mixed rumen bacteria of the sheep and cattle. The contents of total and separate stereoisomers of DAP in Prevotella ruminicola B 4, Streptococcus bovis JB1, Selenomonas 1 ruminantium Z108, Fibrobacter succinogenes S85 and Anaerovibrio lipolytica 5S ranged from 5.68 to 32.54, and from 5.63 to 32.12 μmol g bacterial DM for total DAP and meso-DAP, respectively. LL-DAP was absent -1
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